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Facilities & Equipment

NGS: Next Gen Sequencing Facility

Illumina NextSeq 500 Sequencer
The NextSeq 500 Sequencing System features an exceptional user interface and load-and-go workflows. The NextSeq 500 System integrates cluster generation and sequencing into a single instrument, generating base calls and quality metrics in real time. Sequencing is automated and fast, generating data in less than a day. Data are automatically transferred to the BaseSpace environment for simplified analysis, storage, and sharing.

Agilent 2100 Bioanalyzer
The Agilent 2100 Bioanalyzer system provides sizing, quantitation and quality control of DNA, RNA, proteins and cells on a single platform, providing high quality digital data. Minimal sample input (1-2ul) is required.

StepOne™ Real-Time PCR System
The StepOne™ Real-Time PCR System is a 48-well, low-throughput Real-Time PCR instrument perfect for both first-time and experienced users. The StepOne™ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 3-color optical recording, the StepOne™ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.

Covaris M220 Focused-ultrasonicator
M220 Focused-ultrasonicator™ is designed for Next-Gen Sequencing applications requiring fragment sizes between 150bp and 5kb. Compact size and ease-of-use make the M220 Focused-ultrasonicator the ideal DNA shearing solution for MiSeq™ and PGM™ users. The M220 runs a variety of DNA shearing protocols with Covaris SonoLab 7 software and uses both the Covaris microTUBE™ & miniTUBE™. Integrated temperature control within the M220 eliminates the need for an external chiller, saving space and cost.

Qubit®2.0 Fluorometer
The Qubit®2.0 Fluorometer is a benchtop fluorometer for the quantitation of DNA, RNA, and protein, using the highly sensitive and accurate fluorescence-based Qubit™quantitation assays. Use of the state-of-the-art dyes selective for dsDNA, RNA, and proteinminimizes the effects of contaminants in a sample that affect the quantitation. Further, the very latest illumination and detection technologies used in the Qubit®2.0 Fluorometer for attaining the highest sensitivity allow one to use as little as 1 μL of sample and still achieve high levels of accuracy, even with very dilute samples.

MetaInformatics Laboratory

High Performace Workstations
Several multicore with more than 100 cores on board, high RAM compter servers with more than 2 TB RAM and GPU workstations along with more than 300 TB storage capacity are available in the laboratory for handling the massive computatational requirements of metagenomic, genomic and AIML related analysis.